FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8411964
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG899021.0687397140548867TruSeq Adapter, Index 18 (100% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCC212710.2528660369920746TruSeq Adapter, Index 18 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTATG198430.23589021541223906TruSeq Adapter, Index 18 (97% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGC193580.23012461774681872TruSeq Adapter, Index 18 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGATG104450.1241683868357021TruSeq Adapter, Index 18 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCG102780.12218311918595942TruSeq Adapter, Index 18 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGGATG95840.11393296500080125TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGGATG92380.10981977573846012TruSeq Adapter, Index 18 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)