FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7698905
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG1109861.4415816275171598TruSeq Adapter, Index 16 (97% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC281710.3659091779934939TruSeq Adapter, Index 16 (100% over 39bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT281450.36557146763078646TruSeq Adapter, Index 16 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCGCGTATG187470.24350216037215683TruSeq Adapter, Index 16 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGGATG151090.19624868731332573TruSeq Adapter, Index 16 (97% over 45bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC147580.1916895974167755TruSeq Adapter, Index 16 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCGCGGATG131210.17042683342631193TruSeq Adapter, Index 16 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATGGGGGATG110800.14391656995377916TruSeq Adapter, Index 16 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCGGGGATG105190.13662981943536126TruSeq Adapter, Index 16 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGTATG86330.11213282927896889TruSeq Adapter, Index 16 (97% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)