FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7005754
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG2722013.885391922125727TruSeq Adapter, Index 15 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA593180.8467040093043517TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT264200.37711857995584774TruSeq Adapter, Index 15 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCA229750.3279447151584255TruSeq Adapter, Index 15 (100% over 39bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC160430.2289974783585036TruSeq Adapter, Index 14 (96% over 30bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTC129470.18480523295565332TruSeq Adapter, Index 15 (100% over 38bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT128680.18367758845086482TruSeq Adapter, Index 14 (96% over 29bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAA88930.1269385136846084TruSeq Adapter, Index 14 (96% over 31bp)
AAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA79550.11354951943787919TruSeq Adapter, Index 14 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCGCGTATG79030.11280727242206905TruSeq Adapter, Index 15 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATATCGTATG77850.11112294265542294TruSeq Adapter, Index 15 (97% over 41bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)