FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7327125
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG4894776.680341880341881TruSeq Adapter, Index 14 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA2991644.082965692546531TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT1889272.5784601736697543TruSeq Adapter, Index 8 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTCGTATG1121961.5312417899244246TruSeq Adapter, Index 14 (97% over 41bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCC1073621.4652677550881144TruSeq Adapter, Index 14 (100% over 39bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC734571.0025351007386936TruSeq Adapter, Index 14 (100% over 38bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT563630.769237593189689TruSeq Adapter, Index 14 (97% over 34bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC376250.5135029087124895TruSeq Adapter, Index 14 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCGCGTATG372060.5077844311377245TruSeq Adapter, Index 14 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGGCGTATG319090.43549141034170974TruSeq Adapter, Index 14 (97% over 41bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA282310.38529436852790144TruSeq Adapter, Index 14 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGGATG265150.36187454151526005TruSeq Adapter, Index 14 (97% over 45bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT261430.3567975160789532TruSeq Adapter, Index 14 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGGCGGATG255000.34802190490813245TruSeq Adapter, Index 14 (97% over 41bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAT234330.31981165873381445TruSeq Adapter, Index 2 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTCGGATG221860.3027927051879148TruSeq Adapter, Index 14 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATT216770.29584591500759166TruSeq Adapter, Index 14 (97% over 48bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTG214560.2928297251650545TruSeq Adapter, Index 14 (96% over 33bp)
CGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC201360.27481447361686884Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGGGGGATG178030.2429738812972346TruSeq Adapter, Index 14 (97% over 41bp)
AAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGA176030.24024429772932765TruSeq Adapter, Index 14 (96% over 32bp)
AGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAA172400.23529010355357657TruSeq Adapter, Index 14 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCGCGGATG163260.22281590664824197TruSeq Adapter, Index 14 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCG151580.20687513861166557TruSeq Adapter, Index 14 (97% over 38bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC133280.18189944896531723TruSeq Adapter, Index 14 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTC123240.1681969394544245TruSeq Adapter, Index 14 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGGGGGGTG116600.1591347220089735TruSeq Adapter, Index 14 (97% over 41bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC110810.15123257757988295TruSeq Adapter, Index 14 (96% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTCGTATT108650.1482846273265435TruSeq Adapter, Index 14 (97% over 41bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATC106970.14599177712950168TruSeq Adapter, Index 14 (97% over 38bp)
CACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC105330.14375351860381802Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAG100150.13668389716293908Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTGGTATG91970.12551990037019978TruSeq Adapter, Index 14 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGGGGTATG90600.12365013562618352TruSeq Adapter, Index 14 (97% over 41bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATGTCGTATGC90350.12330893768019516TruSeq Adapter, Index 14 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACAC84180.11488817237320231Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACACG80840.11032976781479775Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACAC80240.10951089274442569Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTTTG78450.10706791545114899TruSeq Adapter, Index 14 (97% over 46bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC78020.106481054984049TruSeq Adapter, Index 14 (97% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)