FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5487457
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG61548311.216179006049614TruSeq Adapter, Index 13 (97% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC1551362.827101879796051TruSeq Adapter, Index 13 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAA902741.6450971734265982TruSeq Adapter, Index 13 (100% over 39bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCA595011.0843091800081532TruSeq Adapter, Index 13 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGATG298570.5440953796995585TruSeq Adapter, Index 13 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATT239730.43686902694636154TruSeq Adapter, Index 13 (97% over 48bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC233200.424969161489557TruSeq Adapter, Index 13 (97% over 48bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAA208690.38030366342733984TruSeq Adapter, Index 13 (97% over 39bp)
CGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC165130.30092263137551695Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATATCGTATG128920.2349357817291325TruSeq Adapter, Index 13 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGTATG122020.22236165130770044TruSeq Adapter, Index 13 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA119140.21711331861005928TruSeq Adapter, Index 14 (97% over 38bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT89470.16304455779790167TruSeq Adapter, Index 14 (96% over 29bp)
CACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC85110.1550991652417504Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGGATG84960.15482581458041494TruSeq Adapter, Index 13 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTTTG78150.1424156945557842TruSeq Adapter, Index 13 (97% over 46bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC77900.14196011012022508TruSeq Adapter, Index 13 (97% over 39bp)
CACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAG77550.1413222919104423Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC75470.13753182940659034TruSeq Adapter, Index 13 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACAC65200.11881642079382125Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTACAGATCGGAAGAGCACAC58570.10673432156279312Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACACG57060.10398259157201598Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCACAGATCGGAAGAGCACAC55950.10195979667813342Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)