FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8094864
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG89311011.033045150604135TruSeq Adapter, Index 12 (100% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG3182933.9320364122238494TruSeq Adapter, Index 12 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA1816852.244447837542422TruSeq Adapter, Index 12 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG1533791.894769325340117TruSeq Adapter, Index 12 (97% over 49bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT1209111.4936754959688021TruSeq Adapter, Index 12 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATT592020.731352620624633TruSeq Adapter, Index 12 (97% over 48bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA469260.5797009066489567TruSeq Adapter, Index 12 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGGATG437610.5406020409978475TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGTATG365860.451965592009946TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGGATG323630.3997967106056383TruSeq Adapter, Index 12 (97% over 45bp)
CGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC315000.38913562970298204Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGTATG262810.32466265029282765TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGATG252500.3119261793650888TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATT249720.30849190301405927TruSeq Adapter, Index 12 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGGATG184640.22809524656621777TruSeq Adapter, Index 12 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTTTG176420.21794065965777806TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGATG170060.21008382599139405TruSeq Adapter, Index 12 (97% over 41bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC169300.2091449590752853TruSeq Adapter, Index 12 (100% over 48bp)
CACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC159710.19729794101543893Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAG148670.18365966370775347Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC145750.1800524381879671TruSeq Adapter, Index 12 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGGTG145480.17971889336250738TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTTTG114540.1414971270672367TruSeq Adapter, Index 12 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGTATG103050.1273029417171184TruSeq Adapter, Index 12 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTAGTATG95810.11835899898997686TruSeq Adapter, Index 12 (97% over 49bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACACG90840.11221930349910758Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAAG89870.11102101282986349TruSeq Adapter, Index 12 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTGGGATG83070.1026206246331007TruSeq Adapter, Index 12 (97% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)