FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5862322
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG108805718.56017120860983TruSeq Adapter, Index 11 (100% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT2209473.7689331974599822TruSeq Adapter, Index 11 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC1331842.271864288587355TruSeq Adapter, Index 11 (100% over 39bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA904711.5432622090700578TruSeq Adapter, Index 11 (100% over 38bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC474740.8098156327816861TruSeq Adapter, Index 11 (100% over 48bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA331270.5650832554063049TruSeq Adapter, Index 11 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATT256730.4379322732528169TruSeq Adapter, Index 11 (97% over 48bp)
CGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC249610.425786915150686Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT183350.3127600292170918TruSeq Adapter, Index 14 (96% over 29bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC154900.26422977107023465TruSeq Adapter, Index 11 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTGTG150110.25605894729085166TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATG148090.2526132136719887TruSeq Adapter, Index 11 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC145290.2478369492498024TruSeq Adapter, Index 11 (100% over 50bp)
CACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC140100.23898380198153565Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC138630.23647626315988785TruSeq Adapter, Index 14 (96% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA136610.23303052954102488TruSeq Adapter, Index 14 (97% over 38bp)
CACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAG125760.21452250490505298Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGT112180.19135762245744947TruSeq Adapter, Index 11 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC103770.17701177110366847TruSeq Adapter, Index 11 (100% over 50bp)
GCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTC92810.15831610750825356TruSeq Adapter, Index 14 (96% over 28bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG79400.13544121254342562TruSeq Adapter, Index 11 (100% over 50bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACACG75510.12880561661403112Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGGATG74820.1276286085957066TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTAAG74510.1270998078918217TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATATCGTATG72090.12297175078407499TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTAGTATG70480.12022539874131787TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACAC67490.11512503066191178Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC62690.1069371487953067TruSeq Adapter, Index 11 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTACAGATCGGAAGAGCACAC61810.10543603711976243Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCACAGATCGGAAGAGCACAC61530.10495841067754381Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTC61400.1047366555436566TruSeq Adapter, Index 11 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)