FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6854067
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC304030.4435760549174672TruSeq Adapter, Index 10 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATG247400.36095357690550733TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG223370.32589410053913975TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATG155670.22712062779660602TruSeq Adapter, Index 10 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT139750.2038935423304149TruSeq Adapter, Index 10 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG132150.19280523519831363TruSeq Adapter, Index 10 (97% over 49bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCT100050.14597172744299114TruSeq Adapter, Index 10 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATG98080.14309752151532806TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTG96270.1404567536325513TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTG79520.1160187083085123TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATG76860.11213780081227687TruSeq Adapter, Index 10 (97% over 45bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)