FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12960805
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG157562012.156806618107439TruSeq Adapter, Index 9 (100% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC2908802.244305041237794TruSeq Adapter, Index 9 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG1827311.409873846570487TruSeq Adapter, Index 9 (100% over 39bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCA1256050.9691141869660103TruSeq Adapter, Index 9 (100% over 38bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC744690.5745707924777821TruSeq Adapter, Index 9 (100% over 48bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA459660.3546538968837198TruSeq Adapter, Index 9 (100% over 40bp)
CGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC305890.23601157489831845Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG290660.22426076158078143TruSeq Adapter, Index 9 (97% over 49bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC271990.20985579213636807TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC253420.1955279783933174TruSeq Adapter, Index 9 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGT225000.1736003280660422TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACAC217850.16808369541861018Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCCAGATCGGAAGAGCACACG200140.1544194207072786Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTACAGATCGGAAGAGCACAC190060.1466421260099199Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC178970.1380855587287981TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGCACAGATCGGAAGAGCACAC162830.12563262852886067Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCC146730.11321056060946831Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG140420.10834203585348287TruSeq Adapter, Index 9 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTC137300.10593477797096708TruSeq Adapter, Index 9 (100% over 50bp)
CACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAG136370.10521722994829412Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)