FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6526906
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTT634100.9715169791015835TruSeq Adapter, Index 8 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATG471540.7224556321172696TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTG282770.4332374328663536TruSeq Adapter, Index 8 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGA271990.4167211845857746TruSeq Adapter, Index 8 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA240120.3678925359121152TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG217170.3327303932368568TruSeq Adapter, Index 8 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTG215970.33089184982900016TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGTATG207170.31740919817138474TruSeq Adapter, Index 8 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTG202500.3102542000758093TruSeq Adapter, Index 8 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATG154220.23628347029971014TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGGATG140410.2151248999142932TruSeq Adapter, Index 8 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGTATG124240.19035052749342493TruSeq Adapter, Index 8 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGATG98350.15068395346891772TruSeq Adapter, Index 8 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGG81610.1250362729293175TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGATT73820.11310106197331476TruSeq Adapter, Index 8 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTT70000.10724836545830443TruSeq Adapter, Index 8 (97% over 42bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAA69240.10608395463332858TruSeq Adapter, Index 8 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATG68620.1051340405392693TruSeq Adapter, Index 8 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)