FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6284592
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG728211.1587227937788165TruSeq Adapter, Index 5 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG568790.9050547752344146TruSeq Adapter, Index 5 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG527370.8391475532540537TruSeq Adapter, Index 5 (97% over 49bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTG352190.5604023300160138TruSeq Adapter, Index 5 (100% over 39bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT195800.3115556268410105TruSeq Adapter, Index 5 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATG160690.2556888338972522TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGGATG122170.19439607217143134TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGGATG95460.1518953020339268TruSeq Adapter, Index 5 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGGGGATG89670.142682293456759TruSeq Adapter, Index 5 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATG67970.1081534012072701TruSeq Adapter, Index 5 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)