FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5790982
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1184652.0456806807550083TruSeq Adapter, Index 4 (100% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC280100.4836830782758434TruSeq Adapter, Index 4 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA153800.26558535322679294TruSeq Adapter, Index 14 (97% over 38bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA136140.2350896618224681TruSeq Adapter, Index 4 (100% over 39bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC82710.14282551733022136TruSeq Adapter, Index 4 (100% over 38bp)
GACAGGTGCCCCGGGTAACCCAGTTCCTCACTATTTTTTACTGCGGAAGC59810.10328127422948301No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)