FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7172692
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1394511.9441933377314962TruSeq Adapter, Index 2 (100% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT1095791.5277248765177704TruSeq Adapter, Index 2 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT563190.7851863707517346TruSeq Adapter, Index 2 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATG518690.7231455079905843TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATG495510.6908284922871357TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGTATG358820.5002584803585599TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA357560.4985018177275701TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATG296800.4137916419665029TruSeq Adapter, Index 2 (97% over 49bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG279940.39028582295182895TruSeq Adapter, Index 2 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGGATG243690.33974691789358863TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGGATG168090.2343471600341964TruSeq Adapter, Index 2 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTG154010.2147171522212302TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGGATG135010.18822779508725596TruSeq Adapter, Index 2 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATT132740.18506301399809164TruSeq Adapter, Index 2 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTG118740.16554454032042643TruSeq Adapter, Index 2 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTT101360.1413137494262963TruSeq Adapter, Index 2 (97% over 42bp)
GATCAATATTAGCCAACTTTAGACAACTCTACGAAACACAAGACAACCCT86570.12069387616253423No Hit
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA85010.11851896052416581TruSeq Adapter, Index 2 (100% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGATT84550.11787763924618538TruSeq Adapter, Index 2 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)