FastQCFastQC Report
Thu 28 Nov 2024
HKN5LAFX7_n01_jz7340_2_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKN5LAFX7_n01_jz7340_2_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7022367
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG5009167.133150403560509TruSeq Adapter, Index 1 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1250151.7802401953643268TruSeq Adapter, Index 14 (97% over 38bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA997641.4206605835325896TruSeq Adapter, Index 1 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACG512090.7292270540688062TruSeq Adapter, Index 1 (100% over 39bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAC290990.4143759504451989TruSeq Adapter, Index 1 (100% over 38bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC172350.24543006652884988TruSeq Adapter, Index 1 (100% over 48bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAT122040.1737875562470603TruSeq Adapter, Index 2 (97% over 37bp)
TCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA121180.17256289795164506TruSeq Adapter, Index 1 (100% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCT84250.11997379231247812TruSeq Adapter, Index 14 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG83330.1186636927406386TruSeq Adapter, Index 1 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC79220.1128109653055729TruSeq Adapter, Index 1 (100% over 50bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCG70720.10070678447879468TruSeq Adapter, Index 14 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)