FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12771866
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC1550711.2141608751610766No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC495200.3877272122961516No Hit
ACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC158390.12401476808478887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT41700.048.2120171
GCCCCCC1103950.046.0277671
GCACCCC27250.039.069021
ACCCCCC85850.034.54581
AGAGCAC150500.026.085018
GGGGGTG96900.022.5114842
GTGGGGG117300.022.3592366
TGGGGGG117400.022.2525967
GGGGTGG120500.021.6475563
GGGTGGG122700.021.3365574
CGTCTGA91500.020.85603516-17
GGTGGGG131250.020.2357145
ACGTCTG94600.020.12263314-15
CGGGGTG21500.019.6728252
CGGAAGA216800.019.1416174
CACGTCT100200.019.0689914-15
ACACGTC101850.018.82989512-13
GAGCACA211900.018.6593539
TCGGAAG223650.018.4494573
GCGGGGT15600.017.6707251
Produced by FastQC (version 0.11.5)