FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_mb284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_mb284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19145557
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT456120.238238041337737TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG74550.037.1460448-49
CGTATGC90150.030.8504446-47
TCGTATG80300.028.92626444-45
ACCCGTC101950.027.2323432-33
CGTCCCG98200.026.82117334-35
CTCGTAT95450.025.87774544-45
GTATGCC108750.024.35094346-47
ATGCCGT109300.024.3148548-49
GTCCCGA119150.023.56019436-37
TGCCGTC126900.022.40235150-51
CGCGTAT15300.022.04275744-45
CCCGATC132150.021.49410838-39
TCTCGTA112300.021.04322642-43
GCCGTCT127200.020.9118350-51
ATCGCGT16900.020.09640542-43
TCCCGAT133350.020.0539936-37
CACCCGT135400.019.92587330-31
CCGTCCC142500.019.81639734-35
TCACCCG147200.019.37728330-31
CCCGTCC142200.018.789332-33
Produced by FastQC (version 0.11.5)