FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_mb283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_mb283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25678580
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT45950.011.1696071
TATGCCG28300.010.40628148-49
CGTATTA14500.010.1552033
CCCGTAT33450.09.8028561
GTCCGTT46200.09.5662261
GTCGGAT37750.09.4415641
GTCCGAA41350.09.3091321
AATCTCG76900.09.14164540-41
GTCCGCT66100.09.058761
GTCGTAT30550.09.0222831
GTTCGTT36700.08.9347551
GTCCGTA29600.08.8302021
CGATCTA14155.456968E-128.3922034
GTCGGTT57250.08.300881
GTCCGCA61950.08.28480151
TCGTATG40850.08.25582444-45
GATCGGA1147650.08.21165294-95
GTTCCGT49600.08.0481731
AGATCGG1211150.08.02035794-95
GCACACG510500.08.00669994-95
Produced by FastQC (version 0.11.5)