FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14865634
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26900.020.48333246-47
TCGTATG23800.020.05773244-45
TATGCCG27500.019.94982548-49
GTCCGTT22550.018.7566551
CGCGTAT5000.016.62496644-45
CGCTAGT5300.016.1401861
TATCGCG5200.015.52863240-41
ATCGCGT5350.015.09342742-43
CTCGTAT32550.015.03069244-45
GTCCGGT19750.014.6782611
CTCCGAA26650.014.6227321
GTCCGAT20550.014.5693651
GTCCGAA18600.014.563771
GTCCGGA25000.014.2571641
CCCGTAT22850.014.1427751
CTCCGGT36450.014.0811491
CTCCGAT34250.013.8755861
CGGGTAT12850.013.6839191
CTCCCGT36450.013.0381011
TATCTCG36650.012.96025840-41
Produced by FastQC (version 0.11.5)