FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei033.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei033.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14589958
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT170590.11692288627561503TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG40700.024.21655748-49
TCGTATG35900.023.5515544-45
CGTATGC42850.023.0573546-47
TATCGCG6250.021.2797640-41
CGCGTAT6700.019.14180644-45
CTCGTAT44650.019.09575744-45
TATCTCG47850.017.91777240-41
GTATGCC56450.017.58649446-47
ATCGCGT7700.016.96429842-43
CTCCGAT31950.016.8086261
GCCGTCT62100.016.10083850-51
CCCGTAT21700.015.7687571
TCCGATA11600.015.5676112
ATGCCGT66150.015.2587148-49
TGCCGTC66700.015.20407650-51
CTCCCGT40050.014.9517751
GTCCGAT18050.014.4813871
GTCCGGT18250.014.3226861
TCTCGTA61350.014.24613842-43
CTCGCGA4402.696288E-814.041561
Produced by FastQC (version 0.11.5)