FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18626870
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT362060.19437511508911587TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG68650.030.89395948-49
CGTATGC71150.029.67539446-47
TCGTATG62300.029.46862844-45
CTCGTAT69950.026.21187444-45
CGCGTAT11050.025.1473444-45
TATCGCG11450.024.89057440-41
TATCTCG77850.023.67348740-41
GCCGTCT90950.023.60632550-51
ATCGCGT11700.023.54727442-43
GTATGCC92900.023.26458446-47
ATGCCGT98700.021.92117748-49
TGCCGTC98250.021.82819650-51
TCTCGTA93300.020.00826342-43
ACGAGTG107450.019.6936332-33
CGAGTGG110850.019.28240832-33
CTCCCGT47900.019.0489311
CCGTCTT115050.018.68197852-53
CTCCGAT43550.018.3326821
ATCTCGT102200.018.28909942-43
GGATATC120100.017.91594338-39
Produced by FastQC (version 0.11.5)