FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16604476
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT365950.22039238094595698TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG65650.032.1973248-49
TCGTATG59150.031.72021744-45
CGTATGC66750.031.45362346-47
CGTACGT73700.028.325434-35
CTCGTAT68300.027.4707344-45
CGTAATC79800.026.36850438-39
GTACGTA80500.026.13921434-35
CCGTACG80450.026.12593832-33
GTATGCC81550.025.94916746-47
TACGTAA81250.025.89792836-37
ACGTAAT81200.025.73838636-37
ACCGTAC82850.025.36912232-33
AATCGCG9000.024.54134640-41
GCCGTCT90750.023.3182350-51
CGCGTAT10000.022.56251144-45
CACCGTA96450.021.93967230-31
ATGCCGT99050.021.62800848-49
TGCCGTC99250.021.60835550-51
AATCTCG102200.018.68370840-41
TCTCGTA103000.018.42355342-43
Produced by FastQC (version 0.11.5)