FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei030.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei030.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14544048
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT434370.298658255253283TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG117000.035.68559332-33
AATCGCG14100.034.52990340-41
TCGTATG42300.033.91258644-45
TTTCGGA126350.032.9696434-35
CTCGTAT64250.032.5292444-45
TATGCCG42300.032.0592548-49
CGTATGC44450.031.0433646-47
TTCGGAA130800.030.99425136-37
GTCACGT135800.030.69269228-29
AGTCACG133950.030.51376328-29
TCGGAAT136250.030.5040136-37
ACGTTTC134100.030.33810432-33
CGGAATC137800.029.78188338-39
GTTTCGG138200.029.7302134-35
AATCTCG132700.027.84833740-41
CACGTTT155950.027.12304530-31
CGTGAAA52050.027.10407362-63
GCGTGAA53750.027.08623160-61
CCGTCGT31200.026.79459452-53
CGCGTAT8300.025.46691144-45
Produced by FastQC (version 0.11.5)