FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei029.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei029.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14913685
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26300.026.63976944-45
TATGCCG31500.025.33306948-49
CGTATGC31700.025.32337846-47
CTCGTAT33200.021.53240844-45
TATCTCG35000.019.9498640-41
TATCGCG5650.017.65474340-41
CGCGTAT5750.017.34784744-45
GTATGCC47600.017.06409846-47
ATCGCGT6200.016.0887342-43
AGTCACG51800.015.72610928-29
GTCCGGT20550.015.4929241
CGCTAGT4702.6011548E-1015.1657471
GTCACGT55100.014.82735728-29
GTCCGTT20450.014.4068411
CCCGTAT20300.014.0451251
TCACGTG62450.013.27241330-31
GCCGTCT61600.013.18571750-51
ATGCCGT64500.012.62971348-49
TGCCGTC66950.012.38035850-51
TCTCGTA60250.012.219933542-43
Produced by FastQC (version 0.11.5)