FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei028.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei028.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16916436
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC766410.4530564239417807TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG103000.038.39249446-47
TCGTATG95950.037.54988542-43
CGTATGC106600.036.98442544-45
CTCGTAT104300.034.93084342-43
GTATGCC123950.031.88414644-45
ACGGCTA130450.030.27642832-33
CGGCTAC129350.030.24012632-33
GCCGTCT136000.029.05873148-49
ATGCCGT138700.028.32228946-47
TGCCGTC143950.027.65187548-49
CGCGTAT12050.026.0168642-43
TCTCGTA141650.025.66947440-41
AGTCACG156100.025.43832828-29
CTACATC162400.024.36382336-37
ATCTCGT152750.024.2239340-41
GTCACGG163650.024.17765428-29
TCACGGC165350.024.14452630-31
CCGTCTT173500.023.01056350-51
CACGGCT176200.022.64428130-31
GCTACAT177650.022.31246834-35
Produced by FastQC (version 0.11.5)