FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei027.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei027.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16668239
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC183840.11029359490225693TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG44550.022.39067346-47
CGTATGC45800.021.62387544-45
TCGTATG40750.021.56438342-43
CTCGTAT47750.018.7015442-43
TATCTCG54600.016.39853938-39
CTCCCGT46600.016.1121831
GTATGCC62800.015.7324644-45
CGCGTAT7350.015.18705242-43
GCCGTCT69650.014.73075148-49
CCCGTAT22850.014.3498061
GTCCGAT21800.013.7330571
CTCCGAT38750.013.4897581
GTCCGTT23050.013.4006411
ATGCCGT76600.013.23928746-47
TGCCGTC80600.012.99468748-49
TATCGCG8700.012.55726338-39
CTCCGGT41500.012.2523351
CGCTAGT5453.3463948E-812.2071621
TCCGATA14850.012.1593862
CTCCGCT68650.012.1138081
Produced by FastQC (version 0.11.5)