FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei026.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei026.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18174460
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC17590999.678961575749707TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATGC1418090.7802652733561272TruSeq Adapter, Index 9 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2976400.063.585219
AGAGCAC3047350.062.147388
AAGAGCA3137250.060.395277
CGGAAGA3214100.059.0093274
GAAGAGC3237000.058.6006746
TCGGAAG3262900.057.9614643
ATCGGAA3314350.056.80632
GGAAGAG3379750.056.2183235
GATCGGA3360250.056.0141221
TATGCCG2071150.045.59694746-47
CGTATGC2084750.045.29696744-45
TCGTATG1929800.045.255542-43
GCCGTCT2087250.045.1432248-49
GTATGCC2094450.045.0656444-45
CTCGTAT1942800.045.0089142-43
TGCCGTC2101150.044.97004348-49
ATGCCGT2102700.044.87664446-47
CCGTCTT2120450.044.4318750-51
CGTCTTC2140550.043.9636150-51
CTTGAAA2134400.043.84251460-61
Produced by FastQC (version 0.11.5)