FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei025.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei025.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11953400
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC126445610.578212056820654TruSeq Adapter, Index 8 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGC979500.8194321280974451TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATTC664480.5558920474509345TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTGC372820.31189452373383303TruSeq Adapter, Index 8 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATGC351560.29410878913112587TruSeq Adapter, Index 8 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2065150.074.62949
AGAGCAC2103750.073.3236168
AAGAGCA2151000.071.728417
CGGAAGA2181000.070.831064
GAAGAGC2206600.069.95556
TCGGAAG2206700.069.870833
ATCGGAA2235450.068.640052
GATCGGA2257950.067.912611
GGAAGAG2292250.067.4395
TATGCCG1448500.046.34905246-47
CGTATGC1453950.046.21106344-45
TCGTATG1357600.046.14233442-43
CTCGTAT1442800.045.9360842-43
GCCGTCT1329650.045.87594648-49
GTATGCC1463900.045.85640744-45
TGCCGTC1457900.045.8135248-49
ATGCCGT1421950.045.6945446-47
CCGTCTT1419550.045.28436350-51
AATCGCG135050.044.91435238-39
ATCTCGT1532150.044.84581440-41
Produced by FastQC (version 0.11.5)