FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei024.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei024.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18331920
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC3452451.8832997307428792TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC267800.14608398902024447TruSeq Adapter, Index 3 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG447800.040.50989546-47
CGTATGC459800.039.4317344-45
TCGTATG427150.039.2542742-43
GCCGTCT470100.038.43641348-49
GTATGCC471900.038.2696744-45
CTCGTAT442050.038.1567942-43
TGCCGTC479500.037.9305748-49
ATGCCGT480450.037.61854646-47
CCGTCTT496650.036.6206750-51
CGTCTTC513800.035.2642750-51
TCTCGTA489200.034.4155940-41
ATCTCGT495050.034.1624240-41
CTTGAAA549600.033.11450660-61
GCTTGAA555250.032.6665158-59
TGCTTGA561700.032.5028158-59
CATCTCG524550.032.07808738-39
CGCGTAT42300.030.20678542-43
AGGGGGG483750.030.17891768-69
TAGGCAT601200.030.0622334-35
TTGAAAA608050.029.79850460-61
Produced by FastQC (version 0.11.5)