FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei023.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei023.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14028995
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1405281.0016968428600908TruSeq Adapter, Index 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG213600.034.88060846-47
CGTATGC226200.032.89565344-45
GCCGTCT224750.032.81129548-49
TCGTATG213150.032.72573542-43
GTATGCC234500.031.87312744-45
TGCCGTC238700.031.36138748-49
ATGCCGT238100.031.2515646-47
CTCGTAT226050.030.84766442-43
CCGTCTT251700.029.53391650-51
CGTCTTC262050.028.14992150-51
AGGGGGG246550.028.02236768-69
TCTCGTA259900.026.9116640-41
ATCTCGT268450.026.15185440-41
ACGATCT271400.025.84097536-37
GATCTCG272850.025.71244238-39
CTTGAAA289500.025.08700660-61
CACGATC299250.025.02332736-37
GCTTGAA293100.024.88430258-59
TCACGAT304850.024.53258534-35
CGATCTC290650.024.2031338-39
Produced by FastQC (version 0.11.5)