FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16480028
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1641970.9963393266079402TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCGCGTAT235370.1428213592840983TruSeq Adapter, Index 19 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG238850.037.92432448-49
CGTATGC248600.036.41883546-47
TCGTATG216950.035.8529344-45
CGCGTAT35600.034.9586244-45
ATCGCGT35600.034.9586242-43
GTATGCC259900.034.79885546-47
GCCGTCT262050.034.43979350-51
CTCGTAT230600.033.8851644-45
TGCCGTC271300.033.60697650-51
ATGCCGT274450.033.1780848-49
CCGTCTT274050.033.13959552-53
TCGCGTA38300.032.24612442-43
CGATCGC39150.031.60586540-41
ACGATCG39550.031.28573638-39
TCTCGTA251050.031.10602842-43
CGTCTTC295500.030.63758552-53
AACGATC298150.030.36499838-39
GATCGCG41750.029.97891640-41
GCGTATG42000.029.46194344-45
ATCTCGT266650.029.3841942-43
Produced by FastQC (version 0.11.5)