FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei021.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei021.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15074303
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1039230.6894050093062346TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCGCGTAT181870.12064902768638788TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG155850.039.83441548-49
CGTATGC161550.038.38558646-47
TCGTATG135550.037.56562844-45
CGCGTAT25450.036.4883644-45
CTCGTAT145750.036.1588144-45
GCCGTCT173050.034.79098550-51
GTATGCC174500.034.5433546-47
TGCCGTC184700.033.81810450-51
ATGCCGT186600.032.30275748-49
ATCGCGT29000.031.93978742-43
CCGTCTT198800.031.40758752-53
CGCACAT199350.029.92669536-37
ATCTCGT178600.029.66767542-43
TCTCGTA174500.029.45284342-43
GCGTATG31750.029.1733544-45
GTCCGCA213250.028.92261334-35
CGTCCGC208150.028.75265932-33
CCGCACA214700.028.7383936-37
CGTCTTC212550.028.34782452-53
ACGTCCG219100.028.25872232-33
Produced by FastQC (version 0.11.5)