FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17600741
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1054460.5990997765378173TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG165850.036.93141648-49
CGTATGC171600.035.72194746-47
TCGTATG151250.034.65538444-45
CGCGTAT25500.033.15687644-45
CTCGTAT161400.032.7261644-45
GCCGTCT187900.032.3572450-51
GTATGCC188700.032.30861746-47
ATCGCGT26550.031.66672542-43
TGCCGTC198750.031.00904850-51
TATCGCG26750.030.98555640-41
ATGCCGT198550.030.70549248-49
TATCTCG173200.030.37270440-41
CCGTCTT202750.030.2684352-53
TCGCGTA28350.029.32104142-43
TCTCGTA184600.028.62613742-43
CCGTATC213600.028.48622138-39
CGTATCT186000.028.15481238-39
TCCGTAT217700.027.76419636-37
ATCTCGT192650.027.56558442-43
CGTCTTC223400.027.37488652-53
Produced by FastQC (version 0.11.5)