FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14500874
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT297310.20502902100935436TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG59450.028.4437448-49
CGTATGC61200.027.74710846-47
TCGTATG53050.027.66726144-45
CTCGTAT61250.024.3509944-45
GTATGCC76100.022.25194446-47
AATCGCG9650.022.1500440-41
CGCGTAT10100.022.10393344-45
GCCGTCT83550.020.43812450-51
ATCGCGT10800.019.79164342-43
ATGCCGT88600.019.46083648-49
AATCTCG76100.019.3493540-41
TGCCGTC90000.019.31644250-51
TCTCGTA79800.018.60116842-43
CCGTCTT95950.018.06912852-53
ATCTCGT88600.016.91448242-43
TCGCGTA13650.016.00730742-43
CGTCTTC115100.015.04220752-53
GTCCGTT19750.014.197151
GCGTATG16150.013.52939544-45
AGTCAAC131150.013.12883134-35
Produced by FastQC (version 0.11.5)