FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei018.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei018.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28120113
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTT69800.020.6973461
CTCCCGT81700.019.2531781
CGCGTTA3305.966285E-1018.7208631
CTCCGAT71400.018.4364811
GTCCGTT31850.017.3079281
CGTATCG32550.016.9278833
CCCGTAT56350.016.0234551
CTCCGGT87800.015.8046491
CTCCGCT75450.015.7462541
GTCCGAT28150.015.7000491
CGCTAGT9750.015.1096211
CTCCGAA63050.014.4714691
GTATCGA37550.014.4207644
CCCGTCT90350.013.8332451
CTCCGTA53700.013.8053291
CCCCGCT74200.013.6418161
CTCGTTT79950.013.25509551
CTCGCGA7900.013.2340291
TCCGATT72000.013.1337882
CTCCGGA88400.013.1169861
Produced by FastQC (version 0.11.5)