FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24297268
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT8900.016.0197491
CCCGTAT48700.014.8333451
CTCCCGT73500.014.2899081
TATGCCG43600.014.27173746-47
CTCCGTT58450.014.2291181
CTCCGAT57900.013.9538751
GTCCGTT31450.013.6002331
TCGTATG42650.013.42023442-43
CTCCGCT84850.013.2745861
CTCCGGT72600.013.0268961
CGTATGC48700.012.77716244-45
CCGCTAT14000.012.5602461
CCCGCAT54950.012.1083461
CCCCGTT50850.011.8696311
CCCCCGT47800.011.7321771
GTCCGAT25700.011.4652371
CTCCGAA56500.011.1873621
CGACCGT13700.011.1007911
CCCCTAT46000.010.8481571
CCCCGGT62050.010.7994551
Produced by FastQC (version 0.11.5)