FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei016.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei016.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23998444
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCGT67300.018.9237171
CTCCGTT61950.018.7936671
CTCCGAT61050.018.3701671
CTCCGGT64850.015.6083291
CTCCGCT70200.015.4342121
CTCCGTA44350.015.4296331
CTCCGAA50450.015.259511
CCCCGTT47150.014.714921
CTCGTTT66750.013.6689991
GTCCGTT28750.013.3885611
CTTCGGT55450.013.1979321
CCCGTCT79100.012.9766921
CTCCTAT122200.012.8719261
CCCGATT32250.012.8196531
CCCGTTT69600.012.6313351
CTCCTTT457550.012.452811
CCCCCGT38200.012.4400861
CCCGTAT45250.012.1822121
CGCCTTT69300.012.1374311
CCCCGCT73900.012.0249681
Produced by FastQC (version 0.11.5)