FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23851272
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGTT58000.020.8923841
CTCCCGT69350.019.5287361
CTCCGAT59800.017.7206421
CTCCGGT73150.015.7858411
CCCGTAT44850.015.3631881
CCCCGTT39250.014.7705251
CTCCGCT63850.014.5871671
CTCGGTT51350.014.3438931
CTCCGGA77200.014.1574921
CTCGTTT70400.014.0399791
CCCGGTT39650.013.7825771
CTCCGAA47750.013.6339911
CGCTAGT7750.013.4895281
TCCGATT58500.013.4023832
GTCCGTT26300.013.3706261
CTCCGTA37700.013.1089461
CTCCTAT126750.013.00941
TCCGGTT43800.012.8015282
CCCCCGT36350.012.68069651
CTCCATT260550.012.5844371
Produced by FastQC (version 0.11.5)