FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei014.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei014.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25123058
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGT8700.016.9332681
CTCCGTT61500.016.0726171
CTCCGAT60950.015.7498341
CTCCCGT71850.014.2864821
GTCCGAT28750.013.55421451
GTCCGTT33850.012.6352011
CTCCGCT85550.012.6096831
CTCCGAA58800.012.123061
CGCTATT17350.011.77788451
CTCCGGT69250.011.7347721
CCCGTAT49700.011.56983951
TCCGATC35850.011.3993692
CGTATCG29900.011.2792833
TCCGATA22850.011.2299822
CGCTAAT12750.011.1817411
CTCCTAT111950.011.1218111
TCCGATT58050.010.9691782
CTCGTAT50400.010.8434041
CCCCTAT49650.010.72005751
TCCGTTA21900.010.6322092
Produced by FastQC (version 0.11.5)