FastQCFastQC Report
Fri 17 Jul 2020
HKMWCDRXX_n01_dei013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMWCDRXX_n01_dei013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19302251
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT26850.023.186831
GTCCGAT24250.021.5573481
GTCCGCT36350.016.3425561
GTCCGGT32700.015.9867171
GTCCGAA32100.015.5452831
GTCCGTA21600.014.7412881
GTTCCGA30650.013.7998351
GTCCATT127450.013.6102911
GTCCCGT38600.013.0506741
GTCCTAT47000.012.8416321
GTCGCAT15150.012.547631
GTCGGAT21100.012.162581
GTCCGAC24250.012.1505061
GTTCCGT32800.011.8810391
GTCACGA33050.011.6473721
GTCCGGA38600.011.450121
GTCGTTA19600.011.3960981
GTCGCTT33700.011.2816981
GTCCTTT207250.011.2590391
GTTACGA14400.011.2209811
Produced by FastQC (version 0.11.5)