FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4602359
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGC253.8741273E-545.0363445
CCTGAGC207.047139E-444.98489813
GTCGCCC3150.040.74716645
TGGTCCC451.9175786E-840.0305643
GTCGCCG5800.037.27145445
TGTCGCC1000.036.02828644
GGTCGCC8750.035.77094344
TGGTCGC10600.033.13850443
GTCCCCC352.808352E-432.16881645
GTAGCCC1500.030.0242345
CCGGTTG300.005128562530.0242345
GTCCCCG300.005128562530.0242345
GGCGCCC751.6916601E-930.0242345
GCCGTTG300.005128562530.0242345
CCCATTA300.005128562530.0242345
CCGTTGG602.4045949E-730.0242345
CGACGCC453.474422E-530.02357344
GTGCCGT300.005129108730.02357344
GTCTCAC300.00512965530.02292343
TAACCCT300.00512965530.02292343