FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n02_2MG_N-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n02_2MG_N-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563511
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAC302.1590022E-645.0136745
GTGGCCC207.019706E-445.0136745
CCGGTGG207.019706E-445.0136745
TGTCGCC253.8812952E-545.0136744
TGTCGCA207.019706E-445.0136744
GTCGCCC1300.045.01366845
CTGTTGT207.022777E-445.00967440
CAGTGGT207.022777E-445.00967440
GTCGCCG1500.043.51321845
GGTCGCC2850.041.85481644
AGGTCGC556.002665E-1140.9178943
TGGTCGC2700.040.84211343
TTGTCGC451.9215804E-840.008643
GTAGCCG356.229613E-638.5831545
CGTACGT356.2524323E-638.56260710
GTAGGCC301.1371475E-437.5113945
GTCGGCC301.1371475E-437.5113945
CGGTGGT3300.036.826140
GTCGCCT250.002103060736.01093745
ATCAGTT250.002104894336.00454338