FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n02_2MG_N-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n02_2MG_N-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences475936
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC351.2071723E-745.0130445
CACGGAG207.01909E-445.01303544
GGTCGCC1250.037.8109544
TAGCACG301.1369756E-437.51086445
GTCGCCG850.037.06955745
CCACAGA250.002109397235.987733
TCGCTGG401.5555644E-533.75977745
TGGTCGC1700.030.44999543
GTAGCAC300.005136979730.00869244
GGTGGCC453.479701E-530.0086944
CGGCCGG300.00513962130.00553942
CCCAGAT300.005152845329.9897774
TCAGGAA602.4211658E-729.9897771
CATACTA300.005152845329.98977711
AACTACT554.1692474E-628.6266049
ACTACTT507.166347E-526.99079710
CAAGACC507.166347E-526.99079713
GGCGGAA1105.456968E-1226.5818461
ACTCGGT450.0012234325.00724243
CGCAGAT450.001227936624.9914784
Produced by FastQC (version 0.11.5)