FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n02_2MG_N-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n02_2MG_N-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073840
Sequences flagged as poor quality0
Sequence length51
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC650.041.56319845
GTCGCCG1300.038.099645
CACGAAG301.14244416E-437.485642
AAATACC250.002111010535.9862177
CTTCAAC250.002111010535.98621713
CCTTTAT250.002111010535.98621713
TGTCGCG352.8104184E-432.16200344
GGTCGCC2500.031.51876344
TGGTCGC2400.030.95448543
GTCGCGC300.005132145730.01786645
GGCGCCC300.005132145730.01786645
TTGTCGC300.005133315530.0164743
CTTTATG453.4986555E-529.98991214
CCTTCAA300.00515675629.98851212
GGTAGCC654.8475886E-727.708844
GTAGCCG450.001222251825.01488945
CGGTGTT450.001222583925.01372340
CCTAGAA450.001228909524.99159214
CTTAGTA551.3716813E-424.53605711
GTCCGAA751.7031398E-623.990811
Produced by FastQC (version 0.11.5)