FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n02_2MG_N-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n02_2MG_N-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909864
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAC207.020581E-445.0155145
TGGACGC207.020581E-445.0155143
GACCAGT207.020581E-445.0155144
ACCAGTA253.8822545E-545.01550745
CTTCTAT207.0434343E-444.98582513
CGCAGAT302.1697033E-644.985824
GTCGCCC1050.042.87191445
GTCGCCG1850.041.36560445
TTGTCGC403.4478217E-739.38857343
GGTCGCC2900.038.80647344
GTAGCCG356.2320923E-638.58471745
GCGCGCC301.1374254E-437.51292442
GGCGCGC301.1374254E-437.51292441
GTAGCCC250.002103318236.01240545
GTCGGCC504.8623406E-836.01240545
CGCCAAA250.002110139535.9886551
TGGTCGC3050.033.9461243
TGTCGCC401.5565342E-533.7616344
CTATCAC352.8178567E-432.14686225
AGGTCGC651.39825715E-831.16458343