FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n02_2MG_N-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n02_2MG_N-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539022
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTCC207.0103904E-445.02544844
CCTCCTT207.0489704E-444.97532310
GTCGCCC659.094947E-1238.10199445
GTCGCCG601.546141E-1037.5246945
GCTGTGG250.002100280336.0203644
CCAGGAA250.002112757235.976921
CCCAGAT250.002112757235.976924
TCCGTGG352.8075348E-432.1640245
GCCGGAA352.829028E-432.122251
TGGTCGC1550.031.95354543
CGCAGAT501.9889667E-631.4798054
TCGTCCT453.500521E-529.9835510
GCTACTG300.005158678629.9835510
GACCGGA300.005161019529.9807661
GCCAGAT300.005161019529.9807663
GGTCGCC1450.029.49943244
TGGCAGA406.185015E-428.106973
ATCGTCC507.178216E-526.9851939
TGCGGAA507.182751E-526.982691
GCAGGAA507.182751E-526.982691
Produced by FastQC (version 0.11.5)