FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n02_2MG_N-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n02_2MG_N-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559820
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCAGA207.0421543E-444.984463
TCAGTGG250.00210052736.0197445
ATCTTCT250.002108835835.99078411
AACCTCC250.002108835835.9907848
GACTAAT250.002108835835.99078410
CCCAGAT250.002109760735.9875684
GGCCTAG250.002109760735.9875682
GGTCGCC702.0008883E-1135.3765344
TCTCCGT352.8124367E-432.1547343
GTCGCCG651.3933459E-831.17092945
GGTGGCC300.005131254430.01644945
TGACAGG300.005135742530.01108443
TTCAGAA300.005153725429.989641
ATTCGGA300.005153725429.989641
CAGATCC300.005153725429.989646
CACAGAT702.8903742E-828.9185814
TGCTTGT554.1682197E-628.62903212
AGGTCGC406.1488576E-428.13539343
TGCGGAA608.2037095E-626.2409341
AGTGCTG450.00122313125.00923541
Produced by FastQC (version 0.11.5)