FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n02_2MG_N-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n02_2MG_N-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757707
Sequences flagged as poor quality0
Sequence length51
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCC253.871817E-545.03481745
CTACTGT253.897569E-544.98429512
GGTCGCC1000.040.52865644
GTCGCCG900.040.03094545
TGGTCGC1300.034.63759243
GCAGGAA1100.032.715851
GCAGTGG352.806709E-432.16772545
GTAGCCG352.806709E-432.16772545
TCGCTGG501.9685212E-631.5243745
GTCGGTC300.005128394330.02122744
GTGTGCA300.00513005130.01924541
ACCGCAA300.005154954329.989532
TCCTATA300.005154954329.9895313
GGCGCAA300.005154954329.989531
CGGACAG453.497614E-529.9895292
GGTAGCC554.139616E-628.65662444
GCACAAA406.1363645E-428.1467645
TGCGGAA755.6528734E-826.9905761
CACGGTG1200.026.26857444
GCGGACA450.001228666524.9912741
Produced by FastQC (version 0.11.5)