FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n02_2MG_N-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n02_2MG_N-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177009
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTGC206.9923827E-445.0322343
GGCCAGA207.0216635E-444.994073
TGGTCGC250.002094926836.02578443
GGTCGCC250.002094926836.02578444
CGGTGGG1350.035.02506645
CACGGAA300.005131852330.0045221
CCGGAAG406.150128E-428.1212922
AGATGTC450.001219371725.01083440
GCTAGAT450.001223398124.9967044
GCCAGAT551.3630456E-424.542224
ACTCGGT751.6736813E-624.0171943
TCTCGGA602.4442025E-422.51611743
GGATCGG1103.9872248E-922.5033911
GGCAGAT602.4582795E-422.4970344
TAGATCC500.002258835122.4970346
CCAGATC903.2586468E-722.4970325
TCGGTGG3450.021.53715345
ACGGGGG854.961721E-621.19163945
GTGGTCG550.00390734620.46919642
AGGAGTT550.00390734620.46919645
Produced by FastQC (version 0.11.5)