FastQCFastQC Report
Fri 26 Apr 2019
HKMVWAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMVWAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4602359
Sequences flagged as poor quality0
Sequence length51
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGGT3650.035.1299971
ACCGGGT1500.034.4931371
CCGGGTT8300.034.4167482
GTGTTTC27050.034.1798861
ACACTCT436450.033.0539131
GTGTGTC8200.030.9999281
TCACTCT144750.030.833241
CCTGGGT300.005156870529.9904463
TGGTACC901.2732926E-1129.9904443
CCCGGGT3250.028.378971
GACTCTT47450.028.2557071
CACGCTT19000.028.056852
GAGTCTT8550.027.8891871
TGGTTCC5350.027.7481692
TGTGTCC6250.027.7111722
GTGGTTC4600.027.3858551
GTCTTTC105450.027.2847541
TGGGTCC2150.027.200642
CCCGCTT5050.027.1695632
GTGTCCC7150.027.054323
Produced by FastQC (version 0.11.5)