HKMVWAFXY_n01_2MG_N-9.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HKMVWAFXY_n01_2MG_N-9.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	563511
Sequences flagged as poor quality	0
Sequence length	51
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCT	1193	0.21170837836351022	Illumina Single End PCR Primer 1 (96% over 30bp)
CTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC	1191	0.211353460713278	Illumina Single End PCR Primer 1 (96% over 26bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC	1115	0.1978665900044542	Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTG	1082	0.19201044877562284	Illumina Single End PCR Primer 1 (96% over 29bp)
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGAA	1049	0.18615430754679144	Illumina Single End PCR Primer 1 (96% over 27bp)
CTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGA	937	0.16627891913378798	Illumina Single End PCR Primer 1 (96% over 28bp)
CTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAA	911	0.1616649896807693	Illumina Single End PCR Primer 1 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCT	905	0.1606002367300727	Illumina Single End PCR Primer 1 (96% over 30bp)
CTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTGAACT	892	0.15829327200356338	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT	881	0.15634122492728625	Illumina Single End PCR Primer 1 (96% over 32bp)
CACTCTTTCCCTACACGACGCTCTTCCGAAGATCGGAAGAGCACACGTCTG	842	0.14942033074775826	Illumina Single End PCR Primer 1 (96% over 29bp)
CACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAA	817	0.14498386011985567	Illumina Single End PCR Primer 1 (96% over 27bp)
ACACTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGA	789	0.14001501301660482	Illumina Single End PCR Primer 1 (96% over 28bp)
CACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTC	764	0.13557854238870226	Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCT	760	0.13486870708823784	Illumina Single End PCR Primer 1 (100% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCTG	719	0.12759289525847764	Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGT	706	0.12528593053196832	Illumina Single End PCR Primer 1 (96% over 32bp)
TCACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTC	697	0.12368880110592341	Illumina Single End PCR Primer 1 (96% over 31bp)
CTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACTCC	663	0.11765520105197591	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCACACGTCTGA	659	0.1169453657515115	Illumina Single End PCR Primer 1 (100% over 28bp)
CTCTTTCCCTACACGACGCTCTTCCAGATCGGAAGAGCACACGTCTGAACT	638	0.11321873042407335	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAAC	584	0.10363595386780383	Illumina Single End PCR Primer 1 (96% over 26bp)
TCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTGAA	574	0.1018613656166428	Illumina Single End PCR Primer 1 (96% over 27bp)
ACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGTCTG	568	0.1007966126659462	Illumina Single End PCR Primer 1 (96% over 29bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGGGT	25	3.8822924E-5	45.011715	1
CTGTCGG	20	7.030429E-4	44.999733	2
CGCATTG	20	7.030429E-4	44.999733	3
CGCGCGT	20	7.030429E-4	44.999733	2
AATATGG	25	3.888402E-5	44.999733	40
GCATTGC	20	7.030429E-4	44.999733	4
GCATTCC	25	3.888402E-5	44.999733	5
AAGGGGG	25	3.888402E-5	44.999733	40
CCCGGTT	20	7.030429E-4	44.999733	2
GTGTGTC	65	0.0	41.549274	1
GTTTTCC	55	6.002665E-11	40.91974	1
TCGGGGT	55	6.002665E-11	40.908848	42
ATCGGGG	160	0.0	40.781006	41
AAGCGGG	45	1.9254003E-8	39.999763	40
TGGGCGG	35	6.231474E-6	38.58147	1
TTTTCGC	35	6.231474E-6	38.58147	1
CGCATTC	35	6.2428753E-6	38.571198	4
GAATATG	35	6.2428753E-6	38.571198	39
GTGTTTC	280	0.0	37.777687	1
ATGGGGG	30	1.139223E-4	37.49978	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content